|
KMID : 0816120010040010034
|
|
Korean Journal of Pediatric Gastroenterolology and Nutrition
2001 Volume.4 No. 1 p.34 ~ p.40
|
|
|
Comparison among Diagnostic Methods of Rotaviral Gastroenteritis in Children
|
|
Lee Jang-Hoon
Ko Eun-Young
Kim Jae-Oong
Lee Jung-Hwa
Baek Lack-Ju
Kim Soon-Kyum
|
|
|
Abstract
|
|
|
Purpose: The purpose of this study was to evaluate the clinical efficacy of reverse transcription-polymerase chain reaction (RT-PCR) in detecting rotaviral gastroenteritis in children comparing with that of commercial immunoassays.
Methods: Stools from 79 children admitted Korea University Hospital due to diarrhea were collected from December 1999 to February 2000. Immunoassays were done using commercial rotavirus Latex kit and Rotatec (ELISA) kit. RT-PCR was performed to amplify group A rotavirus, most commonly pathogenic to human, using VP4- and VP7-specific primers. The detection rates of immunoassays and RT-PCR were compared.
Results: ELISA assay was superior to LA assay and moderately concordant with RT-PCR in detecting rotaviral gastroenteritis.
Conclusion: Although RT-PCR is known very sensitive, it does not have significant advantage over immunoassay in detecting rotaviral gastroenteritis.
|
|
|
KEYWORD
|
|
|
Rotavirus, Gastroenteritis, Diagnosis, RT-PCR, ELISA, Latex agglutination, Child
|
|
|
FullTexts / Linksout information
|
|
 
|
|
|
Listed journal information
|
|
  
|